BORDETELLA PERSUSSIS TESTING
Bordetella pertussis is the causative agent of pertussis (whooping cough). The Bordetella are small aerobic gram negative coccobacilli. Bordetella species, B. parapertussis and B. bronchiseptica can cause disease in humans that resembles pertussis with less severe symptoms. For optimal detection of B. pertussis it is advised that specimens be collected for PCR testing. Collection of a nasopharyngeal swab or nasal aspirate is recommended.
Clinical Case definition: A cough illness lasting at least 2 weeks with one of the following: paroxysms of coughing, inspiratory "whoop," or post-tussive vomiting, without other apparent cause (as reported by a health professional).
Confirmed: A case that is culture positive and in which an acute cough illness of any duration is present; or a case that meets the clinical case definition and is confirmed by positive PCR; or a case that meets the clinical case definition and is epidemiologically linked directly to a case confirmed by either culture or PCR. (Reference: CDC Case Definitions for Reportable Diseases)
Collection of Specimens for PCR.
Complete instructions for collection and submission of samples will be included in each order of Bordetella kits. (Supply Order Form) Submitters may order collection kits to keep on hand.
Materials for Specimen Collection
One (1) polyester nasopharyngeal swab for PCR
Paper Sleeve for Transport of PCR specimen
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Label paper sleeve with patient name, date and source.
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Obtain nasopharyngeal specimen using polyester swab on flexible wire.
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Pass swab through the nose until it touches the posterior nasopharynx.
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This technique should initiate a cough. Allow the swab to remain for a few seconds after the cough.
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Remove the swab and insert into the paper sleeve for PCR analysis.
- Fill out a laboratory requisition slip and mail immediately to the Wyoming Public Health Laboratory.
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Specimens for PCR should be left at room temperature or refrigerated.
Shipment
Bordetella PCR swabs should be submitted with a bacteriology form. Specimens should be shipped as diagnostic specimens according to federal regulations.
Reporting/Interpretation
Positive PCR results will be phoned upon completion of the procedure. A negative result does not preclude the possibility of infection.
Normal value
Negative.
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