WEST NILE VIRUS 

Rob Christensen   777-6064

Beth White         777-7762   

 

The Wyoming Public Health Laboratory uses RT-PCR and ELISA protocols for testing for West Nile Virus.  Both methods look for the presence of antibody to WNV in serum and cerebral spinal fluid (CSF). 

 

Ideal Timing of Specimen Collection

Timing is critical in collection of both CSF and Serum for WNV Testing. False negative test results are possible when specimens are collected too early. 

• CSF specimens for IgM should be collected prior to 8 days post onset of illness. 
• Acute serum specimens should be collected no earlier than 8 days post onset of illness. 
• Convalescent specimens should be collected 2-3 weeks after collection of Acute serum.

Clinical Criteria

• Fever ³  38°C (100.0° C) for 7-10 days.
• Neurological manifestations, including:  altered mental status, altered level of consciousness, agitation, lethargy, change in personality,  headache, and/or neck pain.
• CSF pleocytosis with predominant lymphocytes and/or elevated protein.
• With or without muscle weakness, (especially flaccid paralysis) confirmed by neurological exam or by EMG).

 

 

West Nile Virus

TYPE OF TEST	PCR or ELISA
SPECIMEN REQUIREMENTS
SHIPPING REQUIREMENTS	Ship according to regulations for diagnostic specimens.  Complete and enclose test request form.
DAYS TEST PERFORMED	Daily, Monday through Friday.
RESULTS AVAILABLE	Upon completion of identification.
REPORTING & INTERPRETATION	Positive results are called to the submitter.
ADDITIONAL COMMENTS